Edvotek Exploring the Genetics of Taste Experiment Kit: SNP Analysis of the PTC Gene Using PCR

The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognises the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.

• Group size: for 25 reactions
• Time required: extraction - 30 minutes, PCR setup - 10 minutes, PCR - 2 hours, DNA digest - 60 minutes, electrophoresis - 30 minutes, staining - 5 minutes
• Kit includes: instructions, PCR EdvoBeads™, universal DNA buffer, TE buffer, LyphoPrimer™ mix, LyphoControl™ (complete PCR control), Dryzymes® restriction enzyme HaeIII, restriction enzyme dilution buffer, 100bp ladder, proteinase K, PTC paper, control taste paper, UltraSpec-Agarose™, TBE electrophoresis powder buffer, SYBR® safe stain, disposable plastic cups, 15mL conical tube, microcentrifuge tubes (snap-top), microcentrifuge tubes (1.5mL screw-cap), 0.2mL PCR tubes, and salt packets
• All you need: thermal cycler, 2 x water baths for 55°C and 99°C incubations, horizontal gel electrophoresis apparatus, DC power supply, balance, microcentrifuge, UV or blue light transilluminator, UV safety goggles, automatic micropipettes (5 to 50µL) with tips, microwave or hotplate, pipette pump, 250mL flasks/beakers, hot gloves, disposable gloves, ice buckets and ice, distilled or deionised water, drinking water, and bleach solution
• Storage: some components require freezer storage upon receipt