Edvotek Construction and Cloning of a DNA Recombinant Experiment Kit

Cloning is frequently performed to study gene structure and function, and to enhance gene expression. This experiment is divided into five modules. Clones are constructed by ligation of a vector and a fragment insert. The constructs are then transformed into competent cells and the cells are grown and selected for resistance. Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analyzed by agarose gel electrophoresis. Recommended for college-level courses.

• Group size: for 5 plasmid constructs and analyses
• Time required: complete 5 modules in approximately 5 hours
• Kit includes: instructions, BactoBeads™, enzymes, plasmid DNA, restriction enzyme dilution buffer, enzyme grade water, standard DNA fragments, restriction enzyme reaction buffer, gel loading solution, agarose powder, electrophoresis buffer, SYBR® Safe DNA stain, and calibrated pipette
• You need: electrophoresis apparatus and power supply, automatic micropipette with tips, balance, microwave or hot plate, water bath, large weigh boats for staining, UV transilluminator or blue light visualisation system, floating racks for micro test tubes, pipette pump or bulb, 5 or 10mL pipettes, laboratory glassware, metric rulers, distilled water, and ice
• Storage: some components require freezer and refrigerator storage