Edvotek Transformation of E. coli with pGAL (Blue Colony) Experiment Kit

In this experiment, your students will develop an understanding of bacterial transformation by plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.

• No IPTG used in this experiment
• Explore genetic engineering, the central dogma, and enzymes in this packed experiment
• Transform E. coli with a plasmid DNA containing β-galactosidase and β-lactamase genes
• Identify transformed cells based on their blue color and ability to survive on agar with ampicillin
• Calculate transformation efficiency and compare results to experimental controls
• Group size: for 10 lab groups
• Time required: complete in 50 minutes and grow overnight
• Kit includes: instructions, BactoBeads™, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour™ agar, Petri dishes, sterile pipettes, loops, and microtubes
• All you need: automatic micropipette (5 to 50µL) and tips, two water baths (37°C and 42°C), incubation oven, thermometer, pipette pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves
• Storage: some components require refrigerator and freezer storage