Edvotek Rainbow Transformation Experiment Kit

In this colorful experiment, students will explore the biological process of bacterial transformation using vibrant chromogenic proteins. Students will use three recombinant plasmids to transform E. coli bacteria. The resulting E. coli will be examined for the presence of blue, purple, and pink pigments, as well as for resistance to ampicillin.

• Explore genetic engineering and the central dogma with this colourful experiment
• Transform E. coli with plasmids that contain a gene for ampicillin resistance and a gene for either a blue, purple, or pink pigment
• Select for transformed cells using LB-ampicillin plates and calculate transformation efficiency
• Expose transformed cells to IPTG to demonstrate differential gene expression
• Different plasmids help emphasize the concept of DNA﹥RNA﹥Protein﹥Trait
• Group size: for 10 lab groups
• Time required: set up & plating - 50 minutes, incubation - overnight, transformation efficiency - 15 minutes
• Kit includes: instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ luria broth agar, sterile, recovery broth, Petri plates, sterile pipettes, loops, and microtubes
• All you need: adjustable volume micropipette (5 to 50µL and 50 to 200µL recommended) and tips, two water baths (37°C and 42°C), thermometer, incubation oven (37°C), centrifuge, pipette pumps or bulbs, ice and ice bucket, marking pens, bunsen burner, hot plate or microwave oven, and hot gloves
• Storage: some components require refrigerator and freezer storage