Edvotek DNA Paternity Testing Simulation Experiment Kit

This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents. The experiment does not contain human DNA.

• Learn genetics and molecular biology techniques by solving a hypothetical paternity case
• Introduce students to restriction enzymes, PCR, and electrophoresis
• Perform agarose gel electrophoresis to separate differently-sized DNA molecules
• Load, run, analyse, and size pre-digested PCR samples to determine the biological parents of a child
• Group size: for 8 gels
• Time required: complete in 45 minutes
• Kit includes: instructions, ready-to-load QuickStrip™ DNA samples, UltraSpec-Agarose™, electrophoresis buffer (50X), practice gel loading solution, FlashBlue™ DNA stain
• You will need: horizontal gel electrophoresis apparatus, D.C. power supply, automatic micropipettes with tips, balance, microwave/hot plate/burner, pipette pump, 250mL flasks/beakers, hot gloves, safety goggles, disposable laboratory gloves, small plastic trays/large weight boats (for gel destaining), DNA visualisation system (white light), distilled/deionised water