DNA obtained from a single hair left behind at a crime scene can be used to identify a criminal. In this experiment, your students will compare simulated crime scene DNA with that of two suspects.
- Learn about DNA fingerprinting while solving a crime!
- Introduce the biotechnologies of restriction enzyme digestion, PCR, and electrophoresis
- Perform agarose gel electrophoresis to separate different sized dye molecules
- Load, run, and analyse simulated crime scene and suspect samples
- Group size: for 10 lab groups
- Time required: complete in 45 minutes
- Kit includes: instructions, ready-to-load QuickStrip™ dye samples, UltraSpec-Agarose™, electrophoresis buffer (50X), practice gel loading solution, disposable pipettes
- You will need: horizontal gel electrophoresis apparatus, D.C. power supply, automatic micropipettes with tips (optional), balance, microwave/hot plate/burner, pipette pump, 250mL flasks/beakers, hot gloves, safety goggles, disposable laboratory gloves, visualisation system (white light box), distilled/deionised water
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