Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. It is recommended for use in molecular biology applications at 25-50µg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L.
Mode of Action: Streptomycin acts by inhibiting prokaryote protein synthesis by binding to the S12 protein of the 30S ribosomal subunit, thus preventing the transition from imitation complex to chain-elongating ribosome. This causes miscoding or inhibits initiation.
Mode of Resistance: A mutation in rpsL, a gene for S12 ribosomal protein, prevents binding of streptomycin to the ribosome. An aminoglycoside phosphotransferase also inactivates streptomycin.
- CAS Number: 3810-74-0
- Linear Formula: C21H39N7O12 · 1.5 H2SO4
- Molecular Weight: 728.69
- Beilstein Registry Number: 3894995
- EC Number: 223-286-0
- MDL Number: MFCD00037023
- PubChem Substance ID: 24899705
- Biological Source: Streptomyces griseus
- InChI Key: QTENRWWVYAAPBI-YZTFXSNBSA-N
- Form: Powder
- Potency: =720 I.U. per mg
- Mol Wt: Calculated mol wt 1457.38
- Colour: White to off-white
- Mode of Action: Protein synthesis | interferes
- Antibiotic Activity Spectrum: Gram-negative bacteria, Gram-positive bacteria, Mycobacteria
- Storage Temp.: Solutions can be stored at 2-8°C for up to a month or at -20°C for more extended periods, at 37°C, solutions are stable for three days
- Storage Conditions: Tightly closed container in a dry and well-ventilated place
- Preparation: Stock solutions should be prepared directly in the vial with sterile water at concentrations between 20-25 mg/mL