N1127-1G Display Image

2-Nitrophenyl beta-D-galactopyranoside, >=98% (enzymatic)

Code: N1127-1G 501

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase. β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific ...

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ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase. β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

  • Synonym: o-Nitrophenyl β-D-galactopyranoside, ONPG
  • CAS Number: 369-07-3
  • Empirical Formula (Hill Notation): C12H15NO8
  • Molecular Weight: 301.25
  • Beilstein Registry Number: 92207
  • EC Number: 206-716-1
  • MDL Number: MFCD00063255
  • PubChem Substance ID: 24897461
  • Grade: for molecular biology
  • InChI Key: KUWPCJHYPSUOFW-YBXAARCKSA-N
  • Assay: ≥98% (enzymatic)
  • Form: powder
  • Storage Temp.: −20°C
Synonym
o-Nitrophenyl β-D-galactopyranoside, ONPG
CAS Number
369-07-3
Empirical Formula (Hill Notation)
C12H15NO8
Molecular Weight
301.25
Beilstein Registry Number
92207
EC Number
206-716-1
MDL Number
MFCD00063255
PubChem Substance ID
24897461
Grade
for molecular biology
InChI Key
KUWPCJHYPSUOFW-YBXAARCKSA-N
Assay
≥98% (enzymatic)
Form
powder
Storage Temp.
−20°C