The Colorimeter measures the amount of light penetrating a solution so can be used for investigations which result in a change in colour or opacity e.g. Beer’s law, enzyme kinetics and chemical rate of reaction experiments.
Measuring Colorimetry
It has two ranges: Transmittance (%T) - the amount of light being received referenced to the light sent and Absorbance (Abs) - the amount of light absorbed by the solution.
The light source in the Colorimeter is a white LED which has the advantage of not heating the solution being studied. This white light passes through a cuvette that contains the sample solution, some light is absorbed so the amount received by the photodiode is of lower intensity. A snap-shut lid prevents stray light entering the unit from the environment.
Practical Teaching
The Colorimeter can be used to investigate a number of scientific experiments such as:
- Reaction kinetics
- Reaction kinetics of enzyme systems
- Rate of reaction with Thiosulphate and acid catalyst
- Beer Lamberts law (light absorbance vs. concentration)
- Population growth of micro-organisms
- Estimation of unknowns e.g. glucose concentration, manganese in paper clips
Included is 1 x Colorimeter Sensor, 4 x Colorimeter Slides (Red (630nm), Green (560nm), Amber (600nm) and Blue (470nm)), 5 x Cuvettes with lids
Ranges:
- 0 to 110% transmittance
- 0.0500 to 1.0500 absorbance
Applications include:
- Enzyme concentration versus rates of protein, starch and fat breakdown
- Enzyme inhibition
- Lambert-Beer law
- Acidic breakdown of sodium thiosulphate
- Quantitative analysis of sugar
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