With this educational kit, students use Bio-Rad’s exclusive pGLO plasmid to transform bacteria to express the green fluorescent protein (GFP) gene from the bioluminescent jellyfish Aequorea victoria, which causes the bacteria to glow green under UV light. The same procedure is used to create "designer proteins", which have led to the explosion of new health treatments, agricultural applications, and environmental solutions. Use this kit to illustrate the concept of DNA>RNA>Protein>Trait and the roles that external and internal factors play in gene regulation - all within the exciting context of genetic engineering.
- Hands-on genetic engineering — transform bacteria with a jellyfish gene
- Visible, dramatic phenotype — transformants glow green under UV light
- Teaches gene regulation — GFP expression is under the control of an arabinose-inducible promoter
- Reinforces math skills — students calculate transformation efficiency
- Consumable reagents for kit includes pGLO plasmid, E. coli, nutrient broth, transformation solution, ampicillin, arabinose; education use only